Procr^tm1.1Pcl
Targeted Allele Detail

Summary

• Symbol: Procr^tm1.1Pcl
• Name: protein C receptor, endothelial; targeted mutation 1.1, Patricia C Liaw
• MGI ID: MGI:5775084
• Synonyms: EPCR^R84A
• Gene: Procr Location: Chr2:155593037-155597391 bp, + strand Genetic Position: Chr2, 77.26 cM, cytoband H1-3
• Alliance: Procr^tm1.1Pcl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:225914
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Single point mutation
• Mutation details: Exon 2 was replaced with a modified exon in which a point mutation results in the amino acid substitution of arginine with alanine at position 103 (p.R103A) (or position 84 in the mature protein). Flp-mediated recombination removed the FRT site flanked neomycin resistance cassette inserted downstream of the modified exon 2. This variant lacks the ability to bind to protein C/activated protein C (PC/APC). Western blot analysis confirmed normal protein expression in heart, kidney, liver, lung, and spleen tissue lysates from homozygous mutant mice. (J:225914)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Procr Mutation: 17 strains or lines available

References

• Original: J:225914 Pepler L, et al., Characterization of mice harboring a variant of EPCR with impaired ability to bind protein C: novel role of EPCR in hematopoiesis. Blood. 2015 Jul 30;126(5):673-82
• All: 2 reference(s)