Shank3^tm5.1Gfng
Targeted Allele Detail

Summary

• Symbol: Shank3^tm5.1Gfng
• Name: SH3 and multiple ankyrin repeat domains 3; targeted mutation 5.1, Guoping Feng
• MGI ID: MGI:5775650
• Synonyms: Shank3^fx
• Gene: Shank3 Location: Chr15:89383826-89444464 bp, + strand Genetic Position: Chr15, 44.96 cM
• Alliance: Shank3^tm5.1Gfng page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:229668
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout)
• Mutation: Insertion
• Mutation details: The targeting vector was designed with (from 5' to 3') an inward-facing lox2272:loxP site, an inverted Shank3 PDZ domain (exons 13-16), another inward-facing lox2272:loxP site and an FRT-flanked neomycin resistance cassette all replacing the corresponding endogenous Shank3 sequences. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:229668)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Shank3 Mutation: 85 strains or lines available

References

• Original: J:229668 Mei Y, et al., Adult restoration of Shank3 expression rescues selective autistic-like phenotypes. Nature. 2016 Feb 25;530(7591):481-4
• All: 4 reference(s)