Fustm1c(EUCOMM)Wtsi
Targeted Allele Detail
|
Symbol: |
Fustm1c(EUCOMM)Wtsi |
Name: |
fused in sarcoma; targeted mutation 1c, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:5776262 |
Synonyms: |
FUSFLOX |
Gene: |
Fus Location: Chr7:127565276-127581204 bp, + strand Genetic Position: Chr7, 69.87 cM
|
Alliance: |
Fustm1c(EUCOMM)Wtsi page
|
IMPC: |
Fus gene page |
|
Mutant Cell Line: |
EPD0667_5_C04 |
Germline Transmission: |
Earliest citation of germline transmission:
J:232167
|
Parent Cell Line: |
JM8A3.N1 (ES Cell)
|
Strain of Origin: |
C57BL/6N-Atm1Brd
|
Project Collection: |
EUCOMM
|
|
Allele Type: |
|
Targeted (Conditional ready) |
Mutation: |
|
Insertion
Vector: L1L2_Bact_P
|
|
|
Mutation details: The L1L2_Bact_P cassette was inserted at position 127570465 of Chromosome 7 upstream of the critical exon 4 through 6 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targetedexon 4 through 6 at position 127572092. The critical exon 4 through 6 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml.
(J:232167)
|
|
|
View phenotypes and curated references for all genotypes (concatenated display).
|
Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
Carrying any Fus Mutation: |
46 strains or lines available
|
|
Original: |
J:232167 Sharma A, et al., ALS-associated mutant FUS induces selective motor neuron degeneration through toxic gain of function. Nat Commun. 2016;7:10465 |
All: |
3 reference(s) |
|