Fus^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Fus^{tm1c(EUCOMM)Wtsi}
• Name: fused in sarcoma; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5776262
• Synonyms: FUS^FLOX
• Gene: Fus Location: Chr7:127565276-127581204 bp, + strand Genetic Position: Chr7, 69.87 cM
• Alliance: Fus^{tm1c(EUCOMM)Wtsi} page
• IMPC: Fus gene page

Mutation origin

• Mutant Cell Line: EPD0667_5_C04
• Germline Transmission: Earliest citation of germline transmission: J:232167
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 127570465 of Chromosome 7 upstream of the critical exon 4 through 6 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targetedexon 4 through 6 at position 127572092. The critical exon 4 through 6 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:232167)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fus Mutation: 48 strains or lines available

References

• Original: J:232167 Sharma A, et al., ALS-associated mutant FUS induces selective motor neuron degeneration through toxic gain of function. Nat Commun. 2016;7:10465
• All: 3 reference(s)