Crppa^em2Mbp
Endonuclease-mediated Allele Detail

Summary

• Symbol: Crppa^em2Mbp
• Name: CDP-L-ribitol pyrophosphorylase A; endonuclease-mediated mutation 2, Mouse Biology Program, UC Davis
• MGI ID: MGI:5776276
• Synonyms: Ispd^em2Mbp, Ispd floxed
• Gene: Crppa Location: Chr12:36431449-36739502 bp, + strand Genetic Position: Chr12, 16.84 cM
• Alliance: Crppa^em2Mbp page

Mutation origin

• Strain of Origin: C57BL/6NCrl

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready)
• Mutation: Insertion
• Mutation details: A conditional allele in which the second of the gene's 10 exons is flanked by loxP sites was generated using a variation of CRISPR/Ca9 technology employing paired Cas9 nickase (Cas9n) RNA with associated sgRNAs and a single DNA template containing loxP sites flanking exon 2. Cre recombinase treatment of genomic DNA from an F1 pup inheriting the PCR-verified mutation demonstrated correct excision of exon 2. (J:232151)

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Crppa Mutation: 22 strains or lines available

References

• Original: J:232151 Lee AY, et al., Conditional targeting of Ispd using paired Cas9 nickase and a single DNA template in mice. FEBS Open Bio. 2014;4:637-42
• All: 2 reference(s)