En1^{tm1.1(dreo)Pjen}
Targeted Allele Detail

Summary

• Symbol: En1^{tm1.1(dreo)Pjen}
• Name: engrailed 1; targeted mutation 1.1, Patricia Jensen
• MGI ID: MGI:5776371
• Synonyms: En1^Dre
• Gene: En1 Location: Chr1:120530246-120535719 bp, + strand Genetic Position: Chr1, 52.74 cM, cytoband C2-qter
• Alliance: En1^{tm1.1(dreo)Pjen} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:232241
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• En1^{tm1.1(dreo)Pjen} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	En1 (MGI:95389)

• Mutation details: The mammalian codon-optimized bacteriophage D6 site-specific recombinase gene (dreo) with a 5' a viral 2A oligopeptide (T2A) that mediates ribosomal skipping replaced the stop codon and 41 bp of the 3' UTR in exon 2 without altering the coding sequence. phiC31-mediated recombination removed the neomycin resistance cassette inserted downstream of the recombinase gene. (J:232241)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	En1 (mouse) Summary of all recombinase alleles driven by En1. Alliance mouse En1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any En1 Mutation: 34 strains or lines available

References

• Original: J:232241 Plummer NW, et al., A knock-in allele of En1 expressing dre recombinase. Genesis. 2016 Aug;54(8):447-54
• All: 3 reference(s)