Chd1^tm1.1Alsr
Targeted Allele Detail

Summary

• Symbol: Chd1^tm1.1Alsr
• Name: chromodomain helicase DNA binding protein 1; targeted mutation 1.1, Alexandra Lusser
• MGI ID: MGI:5776692
• Synonyms: Chd1^delta2
• Gene: Chd1 Location: Chr17:15925229-15992872 bp, + strand Genetic Position: Chr17, 8.95 cM
• Alliance: Chd1^tm1.1Alsr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:231724
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutation: Intragenic deletion
• Mutation details: A targeting vector was designed to insert a loxP upstream of exon 2 (containing the start codon), and an FRT-flanked neomycin resistance (neo) cassette, followed by a second loxP site, downstream of exon 2. Cre-mediated recombination removed exon 2 and the selection cassette. Immunoblot analysis of brain, spleen, liver and thymus indicate the presence of an N-terminally truncated protein. (J:231724)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Chd1 Mutation: 93 strains or lines available

References

• Original: J:231724 Piatti P, et al., Embryonic stem cell differentiation requires full length Chd1. Sci Rep. 2015;5:8007
• All: 2 reference(s)