Cdc14a^{tm1d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Cdc14a^{tm1d(EUCOMM)Hmgu}
• Name: CDC14 cell division cycle 14A; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5779859
• Synonyms: Cdc14a^tm1d
• Gene: Cdc14a Location: Chr3:116066202-116222390 bp, - strand Genetic Position: Chr3, 50.24 cM
• Alliance: Cdc14a^{tm1d(EUCOMM)Hmgu} page
• IMPC: Cdc14a gene page

Mutation origin

• Mutant Cell Line: HEPD0623_4_G09
• Germline Transmission: Earliest citation of germline transmission: J:257652
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 116197919 of Chromosome 3 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 3 at position 116198697. The critical exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:257652)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdc14a Mutation: 35 strains or lines available

References

• Original: J:257652 Imtiaz A, et al., CDC14A phosphatase is essential for hearing and male fertility in mouse and human. Hum Mol Genet. 2018 Mar 1;27(5):780-798
• All: 1 reference(s)