Mapre3^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Mapre3^{tm1c(EUCOMM)Wtsi}
• Name: microtubule-associated protein, RP/EB family, member 3; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5780100
• Synonyms: Mapre3^flox
• Gene: Mapre3 Location: Chr5:30972075-31023447 bp, + strand Genetic Position: Chr5, 16.9 cM
• Alliance: Mapre3^{tm1c(EUCOMM)Wtsi} page
• IMPC: Mapre3 gene page

Mutation origin

• Mutant Cell Line: EPD0144_4_G05
• Germline Transmission: Earliest citation of germline transmission: J:224855
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 31019448 of Chromosome 5 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 3 at position 31020181. The critical exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:224855)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mapre3 Mutation: 17 strains or lines available

References

• Original: J:224855 Geyer M, et al., Microtubule-Associated Protein EB3 Regulates IP3 Receptor Clustering and Ca(2+) Signaling in Endothelial Cells. Cell Rep. 2015 Jul 7;12(1):79-89
• All: 1 reference(s)