Gli3^tm3.1Blnw
Targeted Allele Detail

Summary

• Symbol: Gli3^tm3.1Blnw
• Name: GLI-Kruppel family member GLI3; targeted mutation 3.1, Baolin Wang
• MGI ID: MGI:5780889
• Synonyms: Gli3^delta701C
• Gene: Gli3 Location: Chr13:15638308-15904611 bp, + strand Genetic Position: Chr13, 5.43 cM, cytoband A2
• Alliance: Gli3^tm3.1Blnw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:199480
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted upstream of exon 14. An additional loxP site was inserted downstream of exon 14. Flp-mediated recombination removed the selection cassette and left exon 14 floxed. Cre-mediated deletion of exon 14 produces a protein that contains the first 701 residues and 25 additional artificial residues. (J:199480)

Expression

In Mice Carrying this Mutation:	17 assay results
In Structures Affected by this Mutation:	13 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gli3 Mutation: 81 strains or lines available

References

• Original: J:199480 Cao T, et al., Mouse limbs expressing only the Gli3 repressor resemble those of Sonic hedgehog mutants. Dev Biol. 2013 Jul 15;379(2):221-8
• All: 2 reference(s)