Ctsl^tm1.1Thre
Targeted Allele Detail

Summary

• Symbol: Ctsl^tm1.1Thre
• Name: cathepsin L; targeted mutation 1.1, Thomas Reinheckel
• MGI ID: MGI:5780968
• Synonyms: Ctsl^{KiTTC STOP}
• Gene: Ctsl Location: Chr13:64509704-64518586 bp, - strand Genetic Position: Chr13, 33.26 cM
• Alliance: Ctsl^tm1.1Thre page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:216506
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

Allele Type: Targeted (Conditional ready, Null/knockout) Mutations: Insertion, Nucleotide substitutions Mutation details: Exon 2 was replaced with a modified exon 2 in which the conventional start codon M1 ATG was substituted by TTC. A floxed polyadenylation (STOP) sequence was inserted downstream of the modified exon 2. Flp-mediated recombination removed the FRT-flanked puromycin resistance cassette inserted into intron 2. This approach generates a constitutive null allele with the potential to express an M1-mutated cathepsin L mRNA from the endogenous gene locus. (J:216506)

Generation of the Ctsl^tm1.1Thre and Ctsl^tm1.2Thre alleles

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ctsl Mutation: 32 strains or lines available

References

• Original: J:216506 Tholen M, et al., Out-of-frame start codons prevent translation of truncated nucleo-cytosolic cathepsin L in vivo. Nat Commun. 2014;5:4931
• All: 2 reference(s)