Ctsltm1.2Thre
Targeted Allele Detail
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Symbol: |
Ctsltm1.2Thre |
Name: |
cathepsin L; targeted mutation 1.1, Thomas Reinheckel |
MGI ID: |
MGI:5780970 |
Synonyms: |
CtslKiTTCdeltaSTOP, CtslTTCKideltaSTOP |
Gene: |
Ctsl Location: Chr13:64509704-64518586 bp, - strand Genetic Position: Chr13, 33.26 cM
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Alliance: |
Ctsltm1.2Thre page
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Periodical hair loss in Ctsltm1.2Thre/Ctsltm1.2Thre mice
Show the 2 phenotype image(s) involving this allele.
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Germline Transmission: |
Earliest citation of germline transmission:
J:216506
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Parent Cell Line: |
Not Specified (ES Cell)
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Strain of Origin: |
C57BL/6N
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Allele Type: |
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Targeted (Null/knockout) |
Mutations: |
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Insertion, Nucleotide substitutions
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Mutation details: Exon 2 was replaced with a modified exon 2 in which in which the conventional start codon M1 ATG was substituted by TTC. A floxed polyadenylation (STOP) sequence was inserted downstream of the modified exon 2. Flp-mediated recombination removed the FRT-flanked puromycin resistance cassette inserted into intron 2. Cre-mediated recombination removed the floxed polyadenylation sequence and allowed for expression from a transcription initiation site in exon 3. Western blot analysis confirmed the absence of protein expression in kidney, liver and heart lysates from homozygous mutant mice, although mRNA expression levels are comparable to those in wild-type controls as confirmed by RT-PCR.
(J:216506)
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Generation of the Ctsltm1.1Thre and Ctsltm1.2Thre alleles |
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Ctsl Mutation: |
32 strains or lines available
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Original: |
J:216506 Tholen M, et al., Out-of-frame start codons prevent translation of truncated nucleo-cytosolic cathepsin L in vivo. Nat Commun. 2014;5:4931 |
All: |
2 reference(s) |
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