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Ctsltm1.2Thre
Targeted Allele Detail
Summary
Symbol: Ctsltm1.2Thre
Name: cathepsin L; targeted mutation 1.1, Thomas Reinheckel
MGI ID: MGI:5780970
Synonyms: CtslKiTTCdeltaSTOP, CtslTTCKideltaSTOP
Gene: Ctsl  Location: Chr13:64509704-64518586 bp, - strand  Genetic Position: Chr13, 33.26 cM
Alliance: Ctsltm1.2Thre page
Periodical hair loss in Ctsltm1.2Thre/Ctsltm1.2Thre mice

Show the 2 phenotype image(s) involving this allele.

Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:216506
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  C57BL/6N
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Nucleotide substitutions
 
Mutation detailsExon 2 was replaced with a modified exon 2 in which in which the conventional start codon M1 ATG was substituted by TTC. A floxed polyadenylation (STOP) sequence was inserted downstream of the modified exon 2. Flp-mediated recombination removed the FRT-flanked puromycin resistance cassette inserted into intron 2. Cre-mediated recombination removed the floxed polyadenylation sequence and allowed for expression from a transcription initiation site in exon 3. Western blot analysis confirmed the absence of protein expression in kidney, liver and heart lysates from homozygous mutant mice, although mRNA expression levels are comparable to those in wild-type controls as confirmed by RT-PCR. (J:216506)
Generation of the Ctsltm1.1Thre and Ctsltm1.2Thre alleles
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 2 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Ctsl Mutation:  32 strains or lines available
References
Original:  J:216506 Tholen M, et al., Out-of-frame start codons prevent translation of truncated nucleo-cytosolic cathepsin L in vivo. Nat Commun. 2014;5:4931
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory