Col25a1^tm1.1Tiwa
Targeted Allele Detail

Summary

• Symbol: Col25a1^tm1.1Tiwa
• Name: collagen, type XXV, alpha 1; targeted mutation 1.1, Takeshi Iwatsubo
• MGI ID: MGI:5781011
• Gene: Col25a1 Location: Chr3:129973992-130393533 bp, + strand Genetic Position: Chr3, 59.99 cM, cytoband H1
• Alliance: Col25a1^tm1.1Tiwa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:206855
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An FRT-flanked beta-geo cassette replaced exon 2, which contains the furin cleavage site. Flp-mediated recombination removed the beta-geo cassette. Western blot analysis confirmed the absence of protein expression in E18.5 brains. (J:206855)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col25a1 Mutation: 46 strains or lines available

References

• Original: J:206855 Tanaka T, et al., CLAC-P/collagen type XXV is required for the intramuscular innervation of motoneurons during neuromuscular development. J Neurosci. 2014 Jan 22;34(4):1370-9
• All: 1 reference(s)