Atg12^tm1.2Jdth
Targeted Allele Detail

Summary

• Symbol: Atg12^tm1.2Jdth
• Name: autophagy related 12; targeted mutation 1.2, Jayanta Debnath
• MGI ID: MGI:5784707
• Gene: Atg12 Location: Chr18:46865484-46874683 bp, - strand Genetic Position: Chr18, 24.76 cM, cytoband C
• Alliance: Atg12^tm1.2Jdth page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:232686
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 3. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination removed exons 2 and 3. Western blot analysis confirmed the absence of protein expression in mouse embryonic fibroblasts. (J:232686)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Atg12 Mutation: 9 strains or lines available

References

• Original: J:232686 Malhotra R, et al., Loss of Atg12, but not Atg5, in pro-opiomelanocortin neurons exacerbates diet-induced obesity. Autophagy. 2015;11(1):145-54
• All: 1 reference(s)