Gt(ROSA)26Sor^{tm1(CAG-Wnt5a,-AcGFP)Skde}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-Wnt5a,-AcGFP)Skde}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Sudhansu K Dey
• MGI ID: MGI:5787602
• Synonyms: Rosa26^Wnt5a
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-Wnt5a,-AcGFP)Skde} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:233595
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, No functional change, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(CAG-Wnt5a,-AcGFP)Skde} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Wnt5a (MGI:98958)

• Mutation details: The CAG promotor, a loxP-flanked neo-stop cassette, the mouse cDNA and an FRT-flanked IRES2-AcGFP1-Nuc cassette were inserted in intron 1 of the locus. Cre expression is required to excise the neo-stop cassette and allow for expression of the gene and the nucleus-targeted GFP. Flp expression is required to remove the reporter cassette. (J:233595)

Expression

In Mice Carrying this Mutation:	7 assay results
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:233595 Cha J, et al., Appropriate crypt formation in the uterus for embryo homing and implantation requires Wnt5a-ROR signaling. Cell Rep. 2014 Jul 24;8(2):382-92
• All: 5 reference(s)