Mfap4^tm1Ascr
Targeted Allele Detail

Summary

• Symbol: Mfap4^tm1Ascr
• Name: microfibrillar-associated protein 4; targeted mutation 1, Anders Schlosser
• MGI ID: MGI:5787993
• Gene: Mfap4 Location: Chr11:61376257-61379536 bp, + strand Genetic Position: Chr11, 37.96 cM, cytoband B2
• Alliance: Mfap4^tm1Ascr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:232534
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neomycin expression cassette has replaced 1.5 kbp of the 5' non-coding region and the first 3 exons of the gene. RT-PCR analysis detects no gene transcripts and western blot analysis confirms the absence of protein expression in pulmonary tissue of homozygous mutant mice; quantitative ELISA of 2-fold diluted serum from mice detects no protein in their circulation, and immunohistochemical analysis demonstrates absence of the protein in their arterial tissue. (J:233818)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mfap4 Mutation: 24 strains or lines available

References

• Original: J:232534 Holm AT, et al., Characterization of spontaneous air space enlargement in mice lacking microfibrillar-associated protein 4. Am J Physiol Lung Cell Mol Physiol. 2015 Jun 1;308(11):L1114-24
• All: 5 reference(s)