Ppp3cc^tm1.1Osb
Targeted Allele Detail

Summary

• Symbol: Ppp3cc^tm1.1Osb
• Name: protein phosphatase 3, catalytic subunit, gamma isoform; targeted mutation 1.1, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:5788175
• Synonyms: Ppp3cc^{tm1a(EUCOMM)Osb}
• Gene: Ppp3cc Location: Chr14:70455314-70526920 bp, - strand Genetic Position: Chr14, 36.3 cM
• Alliance: Ppp3cc^tm1.1Osb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:226539
• Parent Cell Line: EGR-G101 (ES Cell)
• Strain of Origin: C57BL/6NSlc-Tg(CAG-EGFP,Acr-EGFP)2Osb

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A IKMC vector (design ID:85228) inserted an FRT site, lacZ reporter gene, loxP site, neomycin resistance cassette, FRT site and loxP site upstream of exon 3. An additional loxP site was inserted downstream of exon 3. Cre-mediated recombination removed the selection cassette and exon 3. Western blot analysis confirmed the absence of protein expression in the testis and sperm. (J:226539)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ppp3cc Mutation: 32 strains or lines available

References

• Original: J:226539 Miyata H, et al., Sperm calcineurin inhibition prevents mouse fertility with implications for male contraceptive. Science. 2015 Oct 23;350(6259):442-5
• All: 1 reference(s)