Ppp3r2^em1Osb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ppp3r2^em1Osb
• Name: protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type II); endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:5788176
• Gene: Ppp3r2 Location: Chr4:49678747-49681983 bp, - strand Genetic Position: Chr4, 26.75 cM, cytoband B3
• Alliance: Ppp3r2^em1Osb page

Mutation origin

• Strain of Origin: (C57BL/6NJcl x DBA/2NJcl)F2

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology targeting exon 1 deleted 372 bp of sequence. Western blot analysis confrimed the absence of protein expression in the testis and sperm. (J:226539)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Ppp3r2 Mutation: 14 strains or lines available

References

• Original: J:226539 Miyata H, et al., Sperm calcineurin inhibition prevents mouse fertility with implications for male contraceptive. Science. 2015 Oct 23;350(6259):442-5
• All: 1 reference(s)