Tg(Nkx2-5-tTA,tetO-EGFP/cre)1Smwu
Transgene Detail

Summary

• Symbol: Tg(Nkx2-5-tTA,tetO-EGFP/cre)1Smwu
• Name: transgene insertion 1, Sean Wu
• MGI ID: MGI:5788432
• Synonyms: Nkx2.5 enh-Cre
• Transgene: Tg(Nkx2-5-tTA,tetO-EGFP/cre)1Smwu Location: unknown
• Alliance: Tg(Nkx2-5-tTA,tetO-EGFP/cre)1Smwu page

Transgene origin

• Strain of Origin: C57BL/6NCrl

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(Nkx2-5-tTA,tetO-EGFP/cre)1Smwu expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Nkx2-5 (MGI:97350)

• Mutation details: The transgene was designed with (from 5' to 3') with a mouse NK2 homeobox 5 locus (Nkx2-5) promoter/enhancer sequence (2.1 kbp enhancer fragment from the regulatory region ~9.5 kbp upstream of the translation start site and the 0.5 kbp base promoter sequence), a Tet-Off cassette, an enhanced green fluorescent protein/Cre recombinase fusion gene sequence (eGFP/Cre) and a G-CSF polyA sequence. The Tet-Off cassette contains a tetracycline-regulated transactivator sequence (tTA), 2x polyA sequence and tetracycline-responsive element (TRE with minimal cytomegalovirus promoter CMVmin). (J:230304)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Nkx2-5 (mouse) Summary of all recombinase alleles driven by Nkx2-5. Comparative matrix view of recombinase activities Alliance mouse Nkx2-5 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:230304 Chen WP, et al., Pharmacological inhibition of TGFbeta receptor improves Nkx2.5 cardiomyoblast-mediated regeneration. Cardiovasc Res. 2015 Jan 1;105(1):44-54
• All: 2 reference(s)