Panx3^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Panx3^{tm1c(KOMP)Wtsi}
• Name: pannexin 3; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5788523
• Synonyms: Panx3^fl
• Gene: Panx3 Location: Chr9:37571198-37580518 bp, - strand Genetic Position: Chr9, 20.79 cM
• Alliance: Panx3^{tm1c(KOMP)Wtsi} page
• IMPC: Panx3 gene page

Mutation origin

• Mutant Cell Line: EPD0670_4_C10
• Germline Transmission: Earliest citation of germline transmission: J:233542
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 37577614 of Chromosome 9 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 at position 37578536. The critical exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:233542)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Panx3 Mutation: 23 strains or lines available

References

• Original: J:233542 Moon PM, et al., Deletion of Panx3 Prevents the Development of Surgically Induced Osteoarthritis. J Mol Med (Berl). 2015 Aug;93(8):845-56
• All: 2 reference(s)