Sod2^tm1Smel
Targeted Allele Detail

Summary

• Symbol: Sod2^tm1Smel
• Name: superoxide dismutase 2, mitochondrial; targeted mutation 1, Simon Melov
• MGI ID: MGI:5788667
• Gene: Sod2 Location: Chr17:13226726-13237006 bp, + strand Genetic Position: Chr17, 8.75 cM
• Alliance: Sod2^tm1Smel page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:226565
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The endogenous gene was modified in mouse embryonic stem cells by inserting a PGK/neo cassette between exons 3 and 4. LoxP sites were inserted 3' of exon 1 and 5' of exon 4, such that Cre-mediated recombination will remove exons 2 and 3. Exon 3 is crucial for manganese binding and homotetramer formation, and thus recombination is expected to completely inactivate Sod2. (J:226565)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sod2 Mutation: 26 strains or lines available

References

• Original: J:226565 Velarde MC, et al., Pleiotropic age-dependent effects of mitochondrial dysfunction on epidermal stem cells. Proc Natl Acad Sci U S A. 2015 Aug 18;112(33):10407-12
• All: 1 reference(s)