Fubp1^tm1.1Dlev
Targeted Allele Detail

Summary

• Symbol: Fubp1^tm1.1Dlev
• Name: far upstream element (FUSE) binding protein 1; targeted mutation 1.1, David Levens
• MGI ID: MGI:5789037
• Synonyms: Fubp1^-
• Gene: Fubp1 Location: Chr3:151916059-151942463 bp, + strand Genetic Position: Chr3, 77.05 cM
• Alliance: Fubp1^tm1.1Dlev page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:229985
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted between exons 7 and 8 and a loxP and frt-flanked neomycin selection cassette was inserted between exons 13 and 14. After FLP and Cre-mediated recombination, a deletion of exons 8-13 was created, with a single loxP and a single frt site remaining at the deletion site. This deletion removes K homology (KH) motifs 2, 3 and the amino terminal half of KH4, yielding an allele that is incapable of expressing a protein that binds properly with single-strand nucleic acids. Immunoblot analysis confirmed the absence of protein expression in primary MEFs derived from E13.5 homozygous mutant embryos. (J:229985)

Expression

In Structures Affected by this Mutation:

11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fubp1 Mutation: 54 strains or lines available

References

• Original: J:229985 Zhou W, et al., Far Upstream Element Binding Protein Plays a Crucial Role in Embryonic Development, Hematopoiesis, and Stabilizing Myc Expression Levels. Am J Pathol. 2016 Mar;186(3):701-15
• All: 1 reference(s)