Rtl4^tm1.1Fuis
Targeted Allele Detail

Summary

• Symbol: Rtl4^tm1.1Fuis
• Name: retrotransposon Gag like 4; targeted mutation 1.1, Fumitoshi Ishino
• MGI ID: MGI:5789261
• Gene: Rtl4 Location: ChrX:143471903-143905407 bp, + strand Genetic Position: ChrX, 65.85 cM
• Alliance: Rtl4^tm1.1Fuis page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:228903
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The open reading frame was replaced with a frt-flanked neo cassette and loxP sites flanking the open reading frame in exon 7. The floxed exon 7 region was removed via cre-mediated recombination and the neo cassette via flp-mediated recombination. Lack of the open reading frame was confirmed by RT-PCR, however RT-PCR of the 3-UTR (without the ORF) exhibits an approximate 1.5-fold increase in transcript levels. (J:228903)

Expression

In Mice Carrying this Mutation:

15 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rtl4 Mutation: 7 strains or lines available

References

• Original: J:228903 Irie M, et al., Cognitive Function Related to the Sirh11/Zcchc16 Gene Acquired from an LTR Retrotransposon in Eutherians. PLoS Genet. 2015 Sep;11(9):e1005521
• All: 1 reference(s)