Tg(SFTPC-EGFR*L858R)4-3Kkiu
Transgene Detail

Summary

• Symbol: Tg(SFTPC-EGFR*L858R)4-3Kkiu
• Name: transgene insertion 4-3, Katsuyuki Kiura
• MGI ID: MGI:5789333
• Transgene: Tg(SFTPC-EGFR*L858R)4-3Kkiu Location: unknown
• Alliance: Tg(SFTPC-EGFR*L858R)4-3Kkiu page

Transgene origin

• Strain of Origin: C57BL/6Cr

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(SFTPC-EGFR*L858R)4-3Kkiu expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  EGFR (1956)

  Egfr	(MGI:95294)

• Mutation details: The full-length human cDNA with a T to G mutation at nucleotide 2573 resulting in the substitution of arginine for leucine at codon 858 and also tagged at the COOH-terminal end with FLAG is under the control of the human type II alveolar epithelial cell specific surfactant protein-C promoter. RT-PCR from hemizygous mice shows an approximate 1.5- to 3-fold greater expression of the transgene compared with endogenous Egfr. Western blot confirmed restricted expression to lung tissue. (J:152157)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:152157 Ohashi K, et al., Chemopreventive effects of gefitinib on nonsmoking-related lung tumorigenesis in activating epidermal growth factor receptor transgenic mice. Cancer Res. 2009 Sep 1;69(17):7088-95
• All: 2 reference(s)