Del(1Aim2-Ifi205)1Stsn
Targeted Allele Detail

Summary

• Symbol: Del(1Aim2-Ifi205)1Stsn
• Name: deletion, Chr 1, Daniel Stetson 1
• MGI ID: MGI:5789602
• Synonyms: ALR^-
• Gene: Del(1Aim2-Ifi205)1Stsn Location: unknown Genetic Position: Chr1, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:234263
• Parent Cell Line: iTL IC1 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intergenic deletion, Intragenic deletion
• Del(1Aim2-Ifi205)1Stsn involves 13 genes/genome features (Aim2, Ifi206, Ifi214 ...) View all
• Mutation details: A targeting vector was designed to insert a loxP site and an FRT-flanked hygromycin resistance (hygro) cassette upstream of the first coding exon of the absent in melanoma 2 (Aim2) gene. A second targeting vector was used to insert a second loxP site and an FRT-flanked neomycin resistance (neo) cassette upstream of the first coding exon of the interferon activated gene 205 (Ifi205) gene. Flp-mediated recombination removed the FRT-flanked neo and hygro cassettes. Cre-mediated recombination removed the loxP-flanked region deleting the mouse ALR (Aim2-like receptor) locus, a 570 kilobase region on chromosome 1 spanning 13 genes from Aim2 to Ifi205. The absence of transcript expression was confirmed by RT-PCR on macrophages and embryonic fibroblasts. Western blot confirmed that no protein was expressed in mutant mice. (J:234263)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Del(1Aim2-Ifi205)1Stsn Mutation: 1 strain or line available

References

• Original: J:234263 Gray EE, et al., The AIM2-like Receptors Are Dispensable for the Interferon Response to Intracellular DNA. Immunity. 2016 Aug 16;45(2):255-66
• All: 3 reference(s)