Mir127^tm1.1Acfs
Targeted Allele Detail

Summary

• Symbol: Mir127^tm1.1Acfs
• Name: microRNA 127; targeted mutation 1.1, Anne C Ferguson-Smith
• MGI ID: MGI:5789789
• Synonyms: deltamiR-127/Rtl1, Rtl1^tm1.1Acfs
• Gene: Mir127 Location: Chr12:109559280-109559349 bp, + strand Genetic Position: Chr12, 60.39 cM
• Alliance: Mir127^tm1.1Acfs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:224145
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mir127^tm1.1Acfs involves 1 genes/genome features (Rtl1) View all
• Mutation details: The microRNA sequence was replaced with a floxed neomycin resistance cassette. Cre-mediated recombination removed the selection cassette. The deletion results in a nonsense mutation in exon 3 and a premature stop codon on the opposite strand in Rtl1. Western blot analysis confirmed the absence of Rtl1 protein expression in conceptuses. (J:224145)

Expression

In Mice Carrying this Mutation:

67 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mir127 Mutation: 1 strain or line available

References

• Original: J:224145 Ito M, et al., A trans-homologue interaction between reciprocally imprinted miR-127 and Rtl1 regulates placenta development. Development. 2015 Jul 15;142(14):2425-30
• All: 2 reference(s)