Nars2^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Nars2^em1(IMPC)J
• Name: asparaginyl-tRNA synthetase 2 (mitochondrial)(putative); endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:5790096
• Synonyms: Nars2^-, Nars2^em1J
• Gene: Nars2 Location: Chr7:96600712-96713965 bp, + strand Genetic Position: Chr7, 53.13 cM
• Alliance: Nars2^em1(IMPC)J page
• IMPC: Nars2 gene page

Nars2^em1(IMPC)J/\AlleleSymbol(MGI:5790096|0 mice exhibit embryonic lethality, with embryos recovered at E7.5 but not at E9.5. Embryos are smaller and form a rudimentary egg cylinder but no primitive streak or hallmarks of gastrulation are seen at E7.5.

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele from project Nars2-7918J-F3847 was generated at The Jackson Laboratory by injecting Cas9 RNA and 4 guide sequences CTCTGGCTGACATAAGAGAA, ATTCAGAAAAAGACTGCTAG, GTGAAAATGTTAGACCTTAA and GAACTCTTTAGAAAAGAAAG, which resulted in a 365 bp deletion beginning at Chromosome 7 positive strand position 96,955,866 bp CTAGCAGTCTTTTTCTGAAT, and ending after GAACCAGGAGCCCTTAAGGT at 96,956,230 bp (GRCm38/mm10). This mutation deletes exon 2 and 255 bp of flanking intronic sequence including the splice acceptor and donor, and is predicted to cause a change of amino acid sequence after residue 47 and early truncation 31 amino acids later. There is a single bp (G) insertion 53 bp before the 365 bp deletion that will not alter the results of the deletion. (J:188991)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nars2 Mutation: 15 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 5 reference(s)