Tg(tetO/Prnp-TARDBP*)2Vle
Transgene Detail

Summary

• Symbol: Tg(tetO/Prnp-TARDBP*)2Vle
• Name: transgene insertion 2, Virginia M Y Lee
• MGI ID: MGI:5792270
• Synonyms: tetO-208 TDP-43 Tg
• Transgene: Tg(tetO/Prnp-TARDBP*)2Vle Location: unknown
• Alliance: Tg(tetO/Prnp-TARDBP*)2Vle page

Transgene origin

• Strain of Origin: (C57BL/6J x C3H/HeJ)F1

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(tetO/Prnp-TARDBP*)2Vle expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  TARDBP (23435)

  Tardbp

  (MGI:2387629)

  expression of amino acids 208-414 from human TARDBP

• Mutation details: The transgenic construct contains the Tet-responsive element and mouse prion protein non-coding exons 1-2 upstream of a cDNA sequence encoding amino acids 208-414 of human TAR DNA binding protein (TARDBP or TDP-43) followed by the mouse prion protein non-coding exon 3 and 3' UTR of the moPrP.XhoI vector (including polyadenylation signal). Three founder lines were generated. Founder line 2 had the highest inducible expression of 208 TDP-43 C-terminal fragments (CTFs), and all three lines have the same inducible 208 TDP-43 CTF distribution and biochemical properties. (J:226974)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:226974 Walker AK, et al., An insoluble frontotemporal lobar degeneration-associated TDP-43 C-terminal fragment causes neurodegeneration and hippocampus pathology in transgenic mice. Hum Mol Genet. 2015 Dec 20;24(25):7241-54
• All: 2 reference(s)