Ptchd1^tm2.2Gfng
Targeted Allele Detail

Summary

• Symbol: Ptchd1^tm2.2Gfng
• Name: patched domain containing 1; targeted mutation 2.2, Guoping Feng
• MGI ID: MGI:5792687
• Gene: Ptchd1 Location: ChrX:154356451-154406810 bp, - strand Genetic Position: ChrX, 72.38 cM
• Alliance: Ptchd1^tm2.2Gfng page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:232709
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector containing a FRT-site flanked NEO selection cassette was used to insert loxP sites flanking exon 2. This selection cassette was inserted downstream of exon 2 of the targeted gene, and another loxP site was inserted upstream of exon 2. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination removed exon 2. In situ hybridization confirmed the absence of full-length mRNA in the brain. (J:232709)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ptchd1 Mutation: 11 strains or lines available

References

• Original: J:232709 Wells MF, et al., Thalamic reticular impairment underlies attention deficit in Ptchd1(Y/-) mice. Nature. 2016 Apr 7;532(7597):58-63
• All: 2 reference(s)