Tg(Kap)L49Msgr
Transgene Detail

Summary

• Symbol: Tg(Kap)L49Msgr
• Name: transgene insertion L49, Anna Meseguer
• MGI ID: MGI:5792809
• Synonyms: KAP Tg, L-49, L49, pKAP2-KAP
• Transgene: Tg(Kap)L49Msgr Location: unknown
• Alliance: Tg(Kap)L49Msgr page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Kap)L49Msgr expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Kap (MGI:96653)

• Mutation details: In male mice with this transgene, expression of cDNA encoding the mouse kidney androgen regulated protein (KAP) is driven by the mouse Kap promoter and the distal regulatory sequences of the human angiotensinogen gene (AGT), which together confer androgen responsiveness. The Kap cDNA was cloned into the NotI site of the pKAP2 vector, downstream of Kap nucleotides -1542 through -1 (relative to the transcription initiation site) and followed by the 3' 42 bp of exon 2 (which excludes the translation initiating ATG codon and most of the coding sequence) through exon 5 of human AGT. RT-PCR and western blot analysis of samples from various tissues detects no expression in tissues other than kidney and demonstrates approximately twice the level of both mRNA and protein in kidneys of 6-8 month old male transgenic vs. control mice. Immunohistochemical analysis using an anti-KAP monoclonal antibody showed the protein to be restricted to the proximal tubule cells of the kidney. However, at higher magnification "diffuse, intense staining" was apparent throughout transgenic male mice; control males exhibited less intense general staining. (J:83177, J:95430, J:166496)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Nine transgenic founders were generated, from four of which (L-43, L-49, L-59 and L-64) transgenic lines were derived. Mice of lines L-49 and L-59 underwent detailed characterization. (J:166496)

References

• Original: J:166496 Tornavaca O, et al., Kidney androgen-regulated protein transgenic mice show hypertension and renal alterations mediated by oxidative stress. Circulation. 2009 Apr 14;119(14):1908-17
• All: 6 reference(s)