Ddx60^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ddx60^{tm1b(EUCOMM)Wtsi}
• Name: DExD/H box helicase 60; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5792890
• Gene: Ddx60 Location: Chr8:62381155-62491278 bp, + strand Genetic Position: Chr8, 31.51 cM
• Alliance: Ddx60^{tm1b(EUCOMM)Wtsi} page
• IMPC: Ddx60 gene page

Mutation origin

• Mutant Cell Line: EPD0327_7_A05
• Germline Transmission: Earliest citation of germline transmission: J:228452
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 62407097 of Chromosome 8 upstream of the exon 9 (Build GRCm39; exon 10 in J:228452). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 9 (or exon 10 J:228452) at position 62407909. Cre expression resulted in the deletion of the targeted exons and neomycin selection cassette, creating a reporter knockout mouse. (J:228452)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ddx60 Mutation: 96 strains or lines available

References

• Original: J:228452 Oshiumi H, et al., DDX60 Is Involved in RIG-I-Dependent and Independent Antiviral Responses, and Its Function Is Attenuated by Virus-Induced EGFR Activation. Cell Rep. 2015 May 26;11(8):1193-207
• All: 1 reference(s)