Tg(Dmp1-cre/ERT2)0022Pdp
Transgene Detail

Summary

• Symbol: Tg(Dmp1-cre/ERT2)0022Pdp
• Name: transgene insertion 0022, Paola Divieti Pajevic
• MGI ID: MGI:5792965
• Synonyms: 10kb-Dmp1-Cre-ERT2, Tg(Dmp1-cre/ERT2)1Pdp
• Transgene: Tg(Dmp1-cre/ERT2)0022Pdp Location: unknown
• Alliance: Tg(Dmp1-cre/ERT2)0022Pdp page

Transgene origin

• Strain of Origin: (C57BL/6 x C3H)F1

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Dmp1-cre/ERT2)0022Pdp expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Dmp1 (MGI:94910)

• Mutation details: A fragment (-9624 - +4439) of the mouse Dmp1 gene containing a 9624 bp promoter region, the 95 bp exon 1, 4326 bp intron 1 and 17 bp of initial non-coding region of exon 2 was cloned into a vector in front of the cre/ERT2 cDNA. The linearized vector was injected into B6C3F1 pronuclei. 14 pups that were positive for cre integration were identified and six independent founders were crossed to wild-type B6 mice. Two founders demonstrated penetrant excision of the STOP cassette when crossed to Rosa26-lacZ reporter mice, and were analyzed in greater detail. The two transgenic founder lines (D77 and 0022) were identified to have tamoxifen-inducible Cre recombinase activity in both osteocytes and mature osteoblasts. The two lines were similar with the only difference that line D77 had greater expression in mature osteoblasts. (J:170229)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Dmp1 (mouse) Summary of all recombinase alleles driven by Dmp1. Alliance mouse Dmp1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:170229 Powell WF Jr, et al., Targeted ablation of the PTH/PTHrP receptor in osteocytes impairs bone structure and homeostatic calcemic responses. J Endocrinol. 2011 Apr;209(1):21-32
• All: 12 reference(s)