About   Help   FAQ
Phlpp2tm1d(EUCOMM)Hmgu
Targeted Allele Detail
Summary
Symbol: Phlpp2tm1d(EUCOMM)Hmgu
Name: PH domain and leucine rich repeat protein phosphatase 2; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
MGI ID: MGI:5795604
Gene: Phlpp2  Location: Chr8:110595174-110671303 bp, + strand  Genetic Position: Chr8, 57.33 cM
Alliance: Phlpp2tm1d(EUCOMM)Hmgu page
IMPC: Phlpp2 gene page
Mutation
origin
Mutant Cell Line:  HEPD0619_1_C08
Germline Transmission:  Earliest citation of germline transmission: J:231241
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 110630450 of Chromosome 8 upstream of the critical exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 4 at position 110631337. The critical exon 4 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:231241)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Phlpp2 Mutation:  62 strains or lines available
References
Original:  J:231241 Wen YA, et al., Loss of PHLPP protects against colitis by inhibiting intestinal epithelial cell apoptosis. Biochim Biophys Acta. 2015 Oct;1852(10 Pt A):2013-23
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
11/12/2024
MGI 6.24
The Jackson Laboratory