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Phlpp2tm1d(EUCOMM)Hmgu
Targeted Allele Detail
Summary
Symbol: Phlpp2tm1d(EUCOMM)Hmgu
Name: PH domain and leucine rich repeat protein phosphatase 2; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
MGI ID: MGI:5795604
Gene: Phlpp2  Location: Chr8:110595174-110671303 bp, + strand  Genetic Position: Chr8, 57.33 cM
Alliance: Phlpp2tm1d(EUCOMM)Hmgu page
IMPC: Phlpp2 gene page
Mutation
origin
Mutant Cell Line:  HEPD0619_1_C08
Germline Transmission:  Earliest citation of germline transmission: J:231241
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 110630450 of Chromosome 8 upstream of the critical exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 4 at position 110631337. The critical exon 4 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:231241)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Phlpp2 Mutation:  62 strains or lines available
References
Original:  J:231241 Wen YA, et al., Loss of PHLPP protects against colitis by inhibiting intestinal epithelial cell apoptosis. Biochim Biophys Acta. 2015 Oct;1852(10 Pt A):2013-23
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory