Pnkp^tm1Pmc
Targeted Allele Detail

Summary

• Symbol: Pnkp^tm1Pmc
• Name: polynucleotide kinase 3'- phosphatase; targeted mutation 1, Peter J McKinnon
• MGI ID: MGI:5795742
• Synonyms: Pnkp^Neo
• Gene: Pnkp Location: Chr7:44506563-44512416 bp, + strand Genetic Position: Chr7, 28.94 cM, cytoband B2
• Alliance: Pnkp^tm1Pmc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:226703
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph)
• Mutation: Insertion
• Mutation details: A single loxP site was introduced between exons 7 and 8 and a neomycin selection cassette flanked by frt sites with a single loxP site was inserted between exons 3 and 4. Western blot analysis shows attenuated protein levels. (J:226703)

Expression

In Mice Carrying this Mutation:	7 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pnkp Mutation: 38 strains or lines available

References

• Original: J:226703 Shimada M, et al., Polynucleotide kinase-phosphatase enables neurogenesis via multiple DNA repair pathways to maintain genome stability. EMBO J. 2015 Oct 1;34(19):2465-80
• All: 1 reference(s)