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Variant origin |
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Variant description |
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Notes |
Mapping and Phenotype information for this QTL, its variants and associated markersJ:193231To identify genetic determinants of skin cancer susceptibility and carcinoma development, a large backcross population of FVB/NJcl x (FVB/NJcl x MSM/Ms) F1 mice were subjected to DMBA/TPA-induced skin carcinogenesis. MSM/Ms mice are more resistant to chemically induced skin tumor development than the highly susceptible FVB/N strain. (FVB/NJcl x MSM/Ms) male mice were backcrossed with female FVB/NJcl mice generating 121 F1 Tpr53 homozygous mice, p53+/+. Tpr53 deficient male mice (FVB/NJcl x MSM.Cg-Trp53tm1Sia/+) were backcrossed to female FVB/NJcl mice generating 107 Tpr53 heterozygous F1 backcross mice, p53+/-. Mice were genotyped using PCR assays.Tumor development was monitored for a period of 40 weeks; the number of papillomas at 20 weeks as well as the presence of carcinomas at 40 weeks was documented. In addition papillomas were categorized into three groups based on diameter size, 2mm, 2-6mm and 6mm. All mice were genotyped using 107 SNP markers distributed evenly over the genome.In the FVB/NJcl x (FVB/NJcl x MSM/Ms) F1 backcrossed mice the following significant linkages were detected on Chromosome 7:Stmm1c, skin tumor modifier of MSM 1c, a measure of the total number of papillomas at 20 weeks, LOD=5.109 at 53.6 cM (D7SNP32, rs32219681). Seventy six percent of mice without tumors at this locus were heterzygous with FVB/MSM alleles.Stmm2b, skin tumor modifier of MSM 2b, a measure of the total number of papillomas 2mm in diameter at 20 weeks, LOD=5.063 at 55.0 cM (D7SNP513, rs31348773). Seventy eight percent of mice without tumors at this locus were heterzygous with FVB/MSM alleles.Stmm1d, skin tumor modifier of MSM 1d, a measure of the total number of papillomas measuring between 2 and 6mm in diameter at 20 weeks, LOD=3.921 at 53.6 cM (D7SNP32, rs32219681). Seventy five percent of mice without tumors at this locus were also heterzygous with FVB/MSM alleles. [Table 1.]In the analysis of combined results from both crosses, 121 FVB/NJcl x (FVB/NJcl x MSM/Ms)F1 mice and 107 (FVB/NJcl x (FVB/NJcl x MSM.Cg-Tpr53 |
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References |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 10/29/2024 MGI 6.24 |
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