Trpm2^{tm1.1(icre)Jsmn}
Targeted Allele Detail

Summary

• Symbol: Trpm2^{tm1.1(icre)Jsmn}
• Name: transient receptor potential cation channel, subfamily M, member 2; targeted mutation 1.1, Jan Siemens
• MGI ID: MGI:5795931
• Synonyms: Trpm2-Cre
• Gene: Trpm2 Location: Chr10:77743556-77805746 bp, - strand Genetic Position: Chr10, 39.72 cM
• Alliance: Trpm2^{tm1.1(icre)Jsmn} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:235274
• Parent Cell Line: A9 (ES Cell)
• Strain of Origin: (C57BL/6 x 129P2/OlaHsd)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Trpm2^{tm1.1(icre)Jsmn} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Trpm2 (MGI:1351901)

• Mutation details: A targeting vector containing a T2A viral 2A peptide, iCre recombinase sequence, T2A viral 2A peptide, multicistronic element (derived from insect Thosea asigna virus 2A), and FRT site flanked NEO cassette was inserted upstream of the Trpm2 gene stop codon. The resulting chimeric animals were crossed to FLPeR recombinase (Gt(ROSA)26Sor) expressing females to excise the FRT site flanked NEO cassette. (J:235274)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Trpm2 (mouse) Summary of all recombinase alleles driven by Trpm2. Alliance mouse Trpm2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Trpm2 Mutation: 112 strains or lines available

References

• Original: J:235274 Song K, et al., The TRPM2 channel is a hypothalamic heat sensor that limits fever and can drive hypothermia. Science. 2016 Sep 23;353(6306):1393-1398
• All: 3 reference(s)