Mirc20^tm1.1Blel
Targeted Allele Detail

Summary

• Symbol: Mirc20^tm1.1Blel
• Name: microRNA cluster 20, including Mir302b through Mir367; targeted mutation 1.1, Robert Blelloch
• MGI ID: MGI:5795955
• Synonyms: mir-302-eGFP
• Gene: Mirc20 Location: Chr3:127338877-127339456 bp Genetic Position: Chr3, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:232516
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mirc20^tm1.1Blel involves 4 genes/genome features (Mir302b, Mirc20, Mir302a ...) View all
• Mutation details: The EGFP reporter gene replaced the sequence spanning Mir302a, Mir302b, Mir302c and Mir302d. Cre-mediated recombination removed the floxed neomycin resistance cassette was inserted into the sequence. (J:232516)

Expression

In Mice Carrying this Mutation:	4 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mirc20 Mutation: 1 strain or line available

References

• Original: J:232516 Parchem RJ, et al., miR-302 Is Required for Timing of Neural Differentiation, Neural Tube Closure, and Embryonic Viability. Cell Rep. 2015 Aug 4;12(5):760-73
• All: 3 reference(s)