Meioc^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Meioc^{tm1c(KOMP)Wtsi}
• Name: meiosis specific with coiled-coil domain; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5796294
• Synonyms: Meioc^Flox
• Gene: Meioc Location: Chr11:102556177-102573066 bp, + strand Genetic Position: Chr11, 66.48 cM
• Alliance: Meioc^{tm1c(KOMP)Wtsi} page
• IMPC: Meioc gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:231231
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 102558690 of Chromosome 11 upstream of exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 102559518. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice, resulting in loss of the lacZ sequence and neomycin selection cassette. Exon 3 is thus flanked by loxP sites. (J:231231)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Meioc Mutation: 38 strains or lines available

References

• Original: J:231231 Abby E, et al., Implementation of meiosis prophase I programme requires a conserved retinoid-independent stabilizer of meiotic transcripts. Nat Commun. 2016;7:10324
• All: 1 reference(s)