Whrn^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Whrn^{tm1b(EUCOMM)Wtsi}
• Name: whirlin; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5796356
• Synonyms: Whrn^tm1b
• Gene: Whrn Location: Chr4:63333147-63414228 bp, - strand Genetic Position: Chr4, 33.97 cM, cytoband C1
• Alliance: Whrn^{tm1b(EUCOMM)Wtsi} page
• IMPC: Whrn gene page

Mutation origin

• Mutant Cell Line: EPD0200_4_C04
• Germline Transmission: Earliest citation of germline transmission: J:235390
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Modified isoform(s), Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 63353146 of Chromosome 4 upstream of exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 4 at position 63354342. Cre expression in mice resulted in the removal of the promoter-driven neomycin selection cassette and exon 4, leaving behind the lacZ reporter sequence inserted in intron 3. A reporter knockout mouse was created. This allele expresses the short form of the encoded protein (WHRN-S), but expression of the long form (WHRN-L) is ablated. (J:235390)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Whrn Mutation: 46 strains or lines available

References

• Original: J:235390 Ebrahim S, et al., Alternative Splice Forms Influence Functions of Whirlin in Mechanosensory Hair Cell Stereocilia. Cell Rep. 2016 May 3;15(5):935-43
• All: 1 reference(s)