Igs7^{tm3(tetO-Optopatch3,CAG-tTA)Acoh}
Targeted Allele Detail

Summary

• Symbol: Igs7^{tm3(tetO-Optopatch3,CAG-tTA)Acoh}
• Name: intergenic site 7; targeted mutation 3, Adam Cohen
• MGI ID: MGI:5796839
• Synonyms: Ai155, FLOXed Optopatch-3
• Gene: Igs7 Location: unknown Genetic Position: Chr9, Syntenic
• Alliance: Igs7^{tm3(tetO-Optopatch3,CAG-tTA)Acoh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:349548
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inducible, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: The targeting construct contains a tetO (tetracycline response element; TRE)-loxP-Stop-loxP-Optopatch3-CAG-tTA (tetracycline-controlled transactivator). Optopatch3 is a bicistronic construct that coexpresses QuasAr3-mCitrine (an enhanced archaerhodopsin-3 (Arch)-based voltage indicator) and CheRiff (a sensitive blue-shifted optogenetic actuator) (J:349548)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igs7 Mutation: 66 strains or lines available

References

• Original: J:349548 Adam Y, et al., Voltage imaging and optogenetics reveal behaviour-dependent changes in hippocampal dynamics. Nature. 2019 May;569(7756):413-417
• All: 2 reference(s)