Shank3^tm2.2Yhj
Targeted Allele Detail

Summary

• Symbol: Shank3^tm2.2Yhj
• Name: SH3 and multiple ankyrin repeat domains 3; targeted mutation 2.2, Yong-hui Jiang
• MGI ID: MGI:5800311
• Synonyms: Shank3^deltaex4-22
• Gene: Shank3 Location: Chr15:89383826-89444464 bp, + strand Genetic Position: Chr15, 44.96 cM
• Alliance: Shank3^tm2.2Yhj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:235641
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted into intron 3 and an FRT site flanked neomycin resistance gene cassette and a second loxP site were inserted into intron 9. Subsquently, an FRT site flanked puromycin resistance gene cassette and a third loxP site were inserted downstream of exon 22. Subsequent flp-mediated recombination removed the two selection cassettes and cre-mediated recombination deleted exons 4 to 22, creating a null allele. (J:235641)

Expression

In Structures Affected by this Mutation:

14 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Shank3 Mutation: 85 strains or lines available

References

• Original: J:235641 Wang X, et al., Altered mGluR5-Homer scaffolds and corticostriatal connectivity in a Shank3 complete knockout model of autism. Nat Commun. 2016;7:11459
• All: 5 reference(s)