Aldh3a2^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Aldh3a2^{tm1b(EUCOMM)Wtsi}
• Name: aldehyde dehydrogenase family 3, subfamily A2; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5803882
• Gene: Aldh3a2 Location: Chr11:61114240-61158267 bp, - strand Genetic Position: Chr11, 37.96 cM
• Alliance: Aldh3a2^{tm1b(EUCOMM)Wtsi} page
• IMPC: Aldh3a2 gene page

Vacuolization and broadened intercellular spaces in Aldh3a2^{tm1b(EUCOMM)Wtsi}/Aldh3a2^{tm1b(EUCOMM)Wtsi} epidermis

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: EPD0060_2_D02
• Germline Transmission: Earliest citation of germline transmission: J:234695
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

Allele Type: Targeted (Null/knockout, Reporter) Mutations: Insertion, Intragenic deletion Vector: L1L2_gt0 Mutation details: Cre-mediated excision of the parental Aldh3a2^tm1a(EUCOMM)Wtsi allele resulted in the removal of the floxed critical exon 4, leaving behind the inserted lacZ reporter sequence and the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. RT-PCR and immunoblotting confirmed absence of RNA and protein. (J:234695)

Generation of the Aldh3a2^tm1b(EUCOMM)Wtsi allele

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Aldh3a2 Mutation: 33 strains or lines available

References

• Original: J:234695 Naganuma T, et al., Disruption of the Sjogren-Larsson Syndrome Gene Aldh3a2 in Mice Increases Keratinocyte Growth and Retards Skin Barrier Recovery. J Biol Chem. 2016 May 27;291(22):11676-88
• All: 3 reference(s)