Neil2^tm1.1Tkhz
Targeted Allele Detail

Summary

• Symbol: Neil2^tm1.1Tkhz
• Name: nei like 2 (E. coli); targeted mutation 1.1, Tapas K Hazra
• MGI ID: MGI:5804531
• Gene: Neil2 Location: Chr14:63419892-63431305 bp, - strand Genetic Position: Chr14, 33.24 cM
• Alliance: Neil2^tm1.1Tkhz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:225791
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 2. A neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 2. Cre-mediated recombination removed exon 2 and the selection cassette. Western blot analysis confirmed the absence of protein expression in the kidney. (J:225791)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Neil2 Mutation: 10 strains or lines available

References

• Original: J:225791 Chakraborty A, et al., Neil2-null Mice Accumulate Oxidized DNA Bases in the Transcriptionally Active Sequences of the Genome and Are Susceptible to Innate Inflammation. J Biol Chem. 2015 Oct 9;290(41):24636-48
• All: 3 reference(s)