Olfm4^{tm2.1(EGFP/cre/ERT2)Cle}
Targeted Allele Detail

Summary

• Symbol: Olfm4^{tm2.1(EGFP/cre/ERT2)Cle}
• Name: olfactomedin 4; targeted mutation 2.1, Hans Clevers
• MGI ID: MGI:5805245
• Synonyms: Olfm4-IRES-eGFPCreERT2
• Gene: Olfm4 Location: Chr14:80237742-80260581 bp, + strand Genetic Position: Chr14, 42.78 cM
• Alliance: Olfm4^{tm2.1(EGFP/cre/ERT2)Cle} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:232454
• Parent Cell Line: IB10/E14IB10 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Olfm4^{tm2.1(EGFP/cre/ERT2)Cle} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Olfm4 (MGI:2685142)

• Mutation details: The targeting vector was designed to insert an eGFPCreERT2 (GCE) fusion protein coding sequence and loxp-flanked neo selection cassette into just after the stop codon of the last exon of Olfm4 gene, making use of the endogenous poly A signal. Positive clones were injected into blastocysts to establish the mouse line. The neomycin selection cassette was excised in vivo by crossing the mice with the PGK-Cre mouse strain. (J:232454)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Olfm4 (mouse) Summary of all recombinase alleles driven by Olfm4. Alliance mouse Olfm4 gene page

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Olfm4 Mutation: 30 strains or lines available

References

• Original: J:232454 Schuijers J, et al., Robust cre-mediated recombination in small intestinal stem cells utilizing the olfm4 locus. Stem Cell Reports. 2014 Aug 12;3(2):234-41
• All: 7 reference(s)