Dlg4^{tm1e(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Dlg4^{tm1e(EUCOMM)Wtsi}
• Name: discs large MAGUK scaffold protein 4; targeted mutation 1e, Wellcome Trust Sanger Institute
• MGI ID: MGI:5805884
• Gene: Dlg4 Location: Chr11:69908029-69938107 bp, + strand Genetic Position: Chr11, 42.96 cM, cytoband B4
• Alliance: Dlg4^{tm1e(EUCOMM)Wtsi} page
• IMPC: Dlg4 gene page

Mutation origin

• Mutant Cell Line: EPD0677_5_B02
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 69920875 of Chromosome 11 upstream of exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site downstream of exon 8 in the targeting construct was not found in the final targeted allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:155845, J:173534)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dlg4 Mutation: 46 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 6 reference(s)