Septin8^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Septin8^{tm1c(EUCOMM)Wtsi}
• Name: septin 8; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5806071
• Synonyms: Sept8^flox
• Gene: Septin8 Location: Chr11:53410224-53440432 bp, + strand Genetic Position: Chr11, 31.96 cM
• Alliance: Septin8^{tm1c(EUCOMM)Wtsi} page
• IMPC: Septin8 gene page

Mutation origin

• Mutant Cell Line: EPD0060_3_G04
• Germline Transmission: Earliest citation of germline transmission: J:236408
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 53422307 of Chromosome 11 upstream of exon 2 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of exon 2 at position 53423302. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice, removing the lacZ/neomycin sequence. Exon 2 remains flanked by loxP sites. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:236408)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Septin8 Mutation: 31 strains or lines available

References

• Original: J:236408 Patzig J, et al., Septin/anillin filaments scaffold central nervous system myelin to accelerate nerve conduction. Elife. 2016 Aug 09;5:e17119
• All: 1 reference(s)