Tg(Mt1-TGFBR2*)AM3Epb
Transgene Detail

Summary

• Symbol: Tg(Mt1-TGFBR2*)AM3Epb
• Name: transgene insertion AM3, Erwin P Bottinger
• MGI ID: MGI:5806465
• Synonyms: DNR
• Transgene: Tg(Mt1-TGFBR2*)AM3Epb Location: unknown
• Alliance: Tg(Mt1-TGFBR2*)AM3Epb page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Dominant negative, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Mt1-TGFBR2*)AM3Epb expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  TGFBR2 (7048)

  Tgfbr2

  (MGI:98729)

  dominant negative receptor

• Mutation details: The human sequence between nucleotides -7 to 573, encoding extracellular and transmembrane domains followed by a stop codon at the 3' end, is under the control of the mouse metallothionein 1 promoter and locus control regions. Activity of the promoter is induced with zinc. This results in the expression of a dominant-negative mutant receptor. Northern blot analysis confirmed RNA expression. Two lines were generated, AM3 and BF1. Line AM3 carries three copies per haploid genome and shows highest zinc-induced RNA expression in the pancreas, liver, colon and small intestine. Substantial levels of the dominant negative receptor is expressed in the liver and pancreas without zinc induction. Immunohistochemistry confirmed protein cell-surface expression in acinar cells in the pancreas and hepatocytes in the liver. (J:76993)

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:76993 Bottinger EP, et al., Expression of a dominant-negative mutant TGF-beta type II receptor in transgenic mice reveals essential roles for TGF-beta in regulation of growth and differentiation in the exocrine pancreas. EMBO J. 1997 May 15;16(10):2621-33
• All: 3 reference(s)