Prmt8^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Prmt8^{tm1d(EUCOMM)Wtsi}
• Name: protein arginine N-methyltransferase 8; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:5806495
• Synonyms: prmt8^-
• Gene: Prmt8 Location: Chr6:127665972-127746430 bp, - strand Genetic Position: Chr6, 62.49 cM
• Alliance: Prmt8^{tm1d(EUCOMM)Wtsi} page
• IMPC: Prmt8 gene page

Mutation origin

• Mutant Cell Line: EPD0105_1_A03
• Germline Transmission: Earliest citation of germline transmission: J:234033
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 127688506 of Chromosome 6 upstream of exon 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 5 at position 127689338. A knockout allele was created by flp and cre recombinase expression in mice, removing exon 5, the lacZ sequence and neomycin selection cassette. qRT-PCR analysis indicated that mRNA expression in the cerebellum was decreased by 45% in heterozygous and by >95% in homozygous mutant mice relative to wild-type controls. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:234033)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prmt8 Mutation: 30 strains or lines available

References

• Original: J:234033 Kim JD, et al., PRMT8 as a phospholipase regulates Purkinje cell dendritic arborization and motor coordination. Sci Adv. 2015 Dec;1(11):e1500615
• All: 1 reference(s)