Gt(ROSA)26Sor^{tm1(CAG-GCaMP6f)Khakh}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-GCaMP6f)Khakh}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Baljit Khakh
• MGI ID: MGI:5806654
• Synonyms: Lck-GCaMP6f^flox, R26-Lck-GCaMP6f^flox
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-GCaMP6f)Khakh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:249928
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector is designed with (from 5' to 3') with a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), an FRT site, a loxP-flanked STOP cassette, the GCaMP6f coding sequence with N-terminal Lck tag (described below), a WPRE, a BGH polyA signal, and an attB/attP-flanked PGK-frt-Neo-polyA cassette. Lck-GCaMP6f is a GCaMP6 fast variant calcium indicator with an N-terminal Lck-tag that targets GCaMP6f to the plasma membrane. In the absence of calcium binding, dim EGFP fluorescence is expected as there is a pore from the outside of its barrel into the chromophore. Upon calcium binding, this pore becomes occupied and fluorescence is increased. (J:249928)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1040 strains or lines available

References

• Original: J:249928 Srinivasan R, et al., New Transgenic Mouse Lines for Selectively Targeting Astrocytes and Studying Calcium Signals in Astrocyte Processes In Situ and In Vivo. Neuron. 2016 Dec 21;92(6):1181-1195
• All: 11 reference(s)