Ddr2^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ddr2^{tm1b(EUCOMM)Wtsi}
• Name: discoidin domain receptor family, member 2; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5806884
• Synonyms: Ddr2^lacZ
• Gene: Ddr2 Location: Chr1:169799876-169938331 bp, - strand Genetic Position: Chr1, 76.84 cM, cytoband H1-H5
• Alliance: Ddr2^{tm1b(EUCOMM)Wtsi} page
• IMPC: Ddr2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:236031
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 169825258 of Chromosome 1 upstream of exon 8 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 8 at position 169826185. A knockout reporter allele was created by cre recombinase expression in mice removing exon 8 and the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:236031)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ddr2 Mutation: 61 strains or lines available

References

• Original: J:236031 Corsa CA, et al., The Action of Discoidin Domain Receptor 2 in Basal Tumor Cells and Stromal Cancer-Associated Fibroblasts Is Critical for Breast Cancer Metastasis. Cell Rep. 2016 Jun 14;15(11):2510-23
• All: 7 reference(s)