Tg(CYP19A1-icre)3Fcrt
Transgene Detail

Summary

• Symbol: Tg(CYP19A1-icre)3Fcrt
• Name: transgene insertion 3, Sophie Fouchecourt
• MGI ID: MGI:5807485
• Synonyms: ARO-iCre, aromatase-iCre
• Transgene: Tg(CYP19A1-icre)3Fcrt Location: unknown
• Alliance: Tg(CYP19A1-icre)3Fcrt page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Recombinase)
• Mutation: Insertion
• Tg(CYP19A1-icre)3Fcrt expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  human

  CYP19A1 (1588)

  Cyp19a1

  (MGI:88587)

• Mutation details: The transgene contains the ovary- and testis-specific promoter of the human cytochrome P450, family 19, subfamily a, polypeptide 1 (CYP19A1) gene, comprising 278 base pairs (bp) of 5' flanking DNA and 26 bp of the alternatively spliced exon IIa, driving expression of mammalian codon-improved Cre recombinase (icre); the coding sequence is followed by the SV40 polyadenylation signal. RT-PCR analysis of various tissues (heart, muscle, lung, spleen, brain, liver, pancreas, ovary, testis) demonstrates Cre recombinase-mediated excision of a loxP-flanked sequence only in the ovaries and testes of transgenic mice. Histologic analysis of X-gal-stained testes of mice carrying this transgene and a lacZ Cre reporter allele (Gt(ROSA)26Sor^tm1Sor) reveal Cre recombinase activity from postpartum day 3 (P3) in Sertoli cells at the periphery of the seminiferous tubules and in the Leydig cells of the interstitium, but not in germ cells. (J:229626)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	CYP19A1 (human) Summary of all recombinase alleles driven by CYP19A1. Alliance human CYP19A1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:229626 Fumel B, et al., Expression of dominant-negative thyroid hormone receptor alpha1 in Leydig and Sertoli cells demonstrates no additional defect compared with expression in Sertoli cells only. PLoS One. 2015;10(3):e0119392
• All: 1 reference(s)